Instrumentation

Talos120c

Thermo Scientific Talos120c microscope is operated at 120 keV, equipped with a LaB6 filament and a charge-coupled device, mainly used for sample screening and data collection on negatively stained biological specimens.

 

Glacios

Thermo Scientific Glacios cryo-TEM microscope is featured with a 200 keV XFEG optics, autoloader, Volta phase plate, data automated collection on Falcon III camera. It is applied for both single particle application (SPA) and cryo tomography.

 

Titan Krios

Thermo Scientific Krios G3i cryo-TEM microscope consists of a highly stable 300 keV XFEG optics, autoloader, Volta phase plate, Falcon III camera and a Gatan K3 direct electron detector, making it ideally suited for automated applications of SPA and cryo-tomography.

Aquilos

Thermo Scientific Aquilos cryo-FIB features of a dual beam system – electron beam and ion beam, is designed to the preparation of thin, electron-transparent lamellas for high-resolution cryo-electron tomography, or MicroED of micro-crystals.

 

 

Sample preparation

Our facility is equipped also with glow discharger, carbon coater, Thermo Scientific Vitrobot IV, provides users with access from sample preparation, screening to data collection.

 

Data Processing

Life science department (LIFS) users could send email to yizhang@ust.hk, applying for the access permission to the cryo-EM cluster. Other users shall contact your collaborators at LIFS first.