The Biological Cryo-EM Center is located at the Hong Kong University of Science and Technology, Clear Water Bay. The facility is generously supported by a donation from the Lo Kwee Seong Foundation at UST in 2018. As the first integrated cryo-EM center in Hong Kong, the mission of the facility is to support advanced studies in the field of life science, medical science, biology and other related. We maintain state-of-the-art equipment, provide eligible access for researchers to microscopes and sample preparation equipment, and support researchers in using the equipment successfully. The facility also offers individual hands-on user training courses of sample preparation, using of microscope.
Thermo Scientific Talos120c microscope is operated at 120 keV, equipped with a LaB6 filament and a charge-coupled device, mainly used for sample screening and data collection on negatively stained biological specimens.
Thermo Scientific Glacios cryo-TEM microscope is featured with a 200 keV XFEG optics, autoloader, Volta phase plate, data automated collection on Falcon III camera. It is applied for both single particle application (SPA) and cryo tomography.
Thermo Scientific Krios G3i cryo-TEM microscope consists of a highly stable 300 keV XFEG optics, autoloader, Volta phase plate, Falcon III camera and a Gatan K3 direct electron detector, making it ideally suited for automated applications of SPA and cryo-tomography.
Thermo Scientific Aquilos cryo-FIB features of a dual beam system – electron beam and ion beam, is designed to the preparation of thin, electron-transparent lamellas for high-resolution cryo-electron tomography, or MicroED of micro-crystals.
Our facility is equipped also with glow discharger, carbon coater, Thermo Scientific Vitrobot IV, provides users with access from sample preparation, screening to data collection.
Life science department (LIFS) users could send email to email@example.com, applying for the access permission to the cryo-EM cluster. Other users shall contact your collaborators at LIFS first.
Latest research publications at the Biological Cryo-EM facility
Apicosome: newly identified cell-type-specific organelle in mouse cochlear and vestibular hair cells
- Structural insights into broadly neutralizing antibodies elicited by hybrid immunity against SARS-CoV-2
News & Events
- New schedule for 2021-2022 HKUST Cryo-EM Winter workshop on single particle analysis for academic users
- Latest science output
- A COVID mitigation plan of the Biological Cryo-EM Center, effective from 17 Feb 2022
- 2021-2022 HKUST Cryo-EM Winter workshop on single particle analysis for academic users – Register now
- HKUST Receives HK$100 million Donation from Lo Kwee Seong Foundation to Advance Frontiers of Knowledge of Life Science
- HKUST Scientists Determine Atomic Structure of DNA Replication Machine to Make Groundbreaking Discovery of DNA Replication Mechanism
Any use of the resources at HKUST Biological Cryo-EM center requires acknowledgment of use of the resources in publications as follows:
“The EM dataset was collected at the Biological Cryo-EM Center, generously supported by a donation from the Lo Kwee Seong Foundation, to UST”.