Publications

Latest research publications at the Biological Cryo-EM facility

• Distinct roles of MCM2-7 subunits in replication licensing in human cells

• DNA-guided CRISPR–Cas12a effectors for programmable RNA recognition and cleavage

• DICER cleavage fidelity is governed by 5′-end binding pockets

• A bispecific antibody designed to act as a NRP2/PLXNA1 agonist mimics anti-cancer activity of SEMA3F

• Capturing the native structure of membrane proteins using vesicles

• H2B.W2, a spermatocyte-specific histone variant, disrupts nucleosome stability, and reduces chromatin compaction

• Tubulin isotypes of C. elegans harness the mechanosensitivity of the lattice for microtubule luminal accessibility

• DNA bending mediated by ORC is essential for replication licensing in budding yeast

• Tubulin acetyltransferases access and modify the microtubule luminal K40 residue through anchors in taxane-binding pockets

• Ultrapotent class I neutralizing antibodies post Omicron breakthrough infection overcome broad SARS-CoV-2 escape variants

• Testis-specific H2B.W1 disrupts nucleosome integrity by reducing DNA–histone interactions

• Assembly and activation of replicative helicases at origin DNA for replication initiation

• Modular Structure and Polymerization Status of GABA_A Receptors Illustrated with EM Analysis and AlphaFold2 Prediction

• Replication licensing regulated by a short linear motif within an intrinsically disordered region of origin recognition complex

• The Origin Recognition Complex: From Origin Selection to Replication Licensing in Yeast and Humans

• Characterization of extracellular vesicles released from Prochlorococcus MED4 at the steady state and under a light–dark cycle

• High-Performance Gel-Free and Label-Free Size Fractionation of Extracellular Vesicles with Two-Dimensional Electrophoresis in a Microfluidic Artificial Sieve

• Structure and assembly of the α-carboxysome in the marine cyanobacterium Prochlorococcus

• Parental histone transfer caught at the replication fork

• Cryo-EM structure of TMEM63C suggests it functions as a monomer

• The Novel H2B Variant, H2BW2, Destabilizes Nucleosomes and Regulates Gene Expression During Early Human Spermatogenesis

 

• Cryo-EM structure of cyanophage P-SCSP1u offers insights into DNA gating and evolution of T7-like viruses

• Metallo-supramolecular branched polymer protects particles from air-water interface in single-particle cryo-electron microscopy

• Menin “reads” H3K79me2 mark in a nucleosomal context

• DDK promotes DNA replication initiation: Mechanistic and structural insights

• Structural insights into broadly neutralizing antibodies elicited by hybrid immunity against SARS-CoV-2

• Apicosome: newly identified cell-type-specific organelle in mouse cochlear and vestibular hair cells

• The human pre-replication complex is an open complex

• Synergism between CMG helicase and leading strand DNA polymerase at replication fork

• Abundant and cosmopolitan lineage of cyanopodoviruses lacking a DNA polymerase gene

• Biochemical and structural characterization of the cyanophage-encoded phosphate-binding protein: implications for enhanced phosphate uptake of infected cyanobacteria

• Structural insights into broadly neutralizing antibodies elicited by hybrid immunity against SARS-CoV-2

• Cryo-EM structure of human hexameric MCM2-7 complex

• A broadly neutralizing antibody protects Syrian hamsters against SARS-CoV-2 Omicron challenge

• Recent Technical Advances in Sample Preparation for Single-Particle Cryo-EM

• Structural Insight into the MCM double hexamer activation by Dbf4-Cdc7 kinase