The Biological Cryo-EM Center is located at the Hong Kong University of Science and Technology, Clear Water Bay. The facility is generously supported by a donation from the Lo Kwee Seong Foundation at UST in 2018. As the first integrated cryo-EM center in Hong Kong, the mission of the facility is to support advanced studies in the field of life science, medical science, biology and other related. We maintain state-of-the-art equipment, provide eligible access for researchers to microscopes and sample preparation equipment, and support researchers in using the equipment successfully. The facility also offers individual hands-on user training courses of sample preparation, using of microscope.
Instrumentation
Talos120c
Thermo Scientific Talos120c microscope is operated at 120 keV, equipped with a LaB6 filament and a charge-coupled device, mainly used for sample screening and data collection on negatively stained biological specimens.
Glacios
Thermo Scientific Glacios cryo-TEM microscope is featured with a 200 keV XFEG optics, autoloader, Volta phase plate, data automated collection on Falcon III camera. It is applied for both single particle application (SPA) and cryo tomography.
Titan Krios
Thermo Scientific Krios G3i cryo-TEM microscope consists of a highly stable 300 keV XFEG optics, autoloader, Volta phase plate, Falcon III camera and a Gatan K3 direct electron detector, making it ideally suited for automated applications of SPA and cryo-tomography.
Aquilos
Thermo Scientific Aquilos cryo-FIB features of a dual beam system – electron beam and ion beam, is designed to the preparation of thin, electron-transparent lamellas for high-resolution cryo-electron tomography, or MicroED of micro-crystals.
Sample preparation
Our facility is equipped also with glow discharger, carbon coater, Thermo Scientific Vitrobot IV, provides users with access from sample preparation, screening to data collection.
Data Processing
Life science department (LIFS) users could send email to yizhang@ust.hk, applying for the access permission to the cryo-EM cluster. Other users shall contact your collaborators at LIFS first.
Publications
Latest research publications at the Biological Cryo-EM facility
Acknowledgements
Any use of the resources at HKUST Biological Cryo-EM center requires acknowledgment of use of the resources in publications as follows:
“The EM dataset was collected at the Biological Cryo-EM Center, generously supported by a donation from the Lo Kwee Seong Foundation, to UST”.